The mushroom cultivator. A practical guide to growing mushrooms at home

Paul Stamets. The mushroom cultivator. A practical guide to growing mushrooms at home. - Agarikon press, 1983

Содержание

FOREWORD by Dr. Andrew Weil

PREFACE

I. INTRODUCTION TO MUSHROOM CULTURE

II. STERILE TECHNIQUE AND AGAR CULTURE

III. GRAIN CULTURE

IV. THE MUSHROOM GROWING ROOM

V. COMPOST PREPARATION

VI. NON-COMPOSTED SUBSTRATES

VII. SPAWNING AND SPAWN RUNNING IN BULK SUBSTRATES

VIII. THE CASING LAYER

IX. STRATEGIES FOR MUSHROOM FORMATION (PINHEAD INITIATION)

X. ENVIRONMENTAL FACTORS: SUSTAINING THE MUSHROOM CROP

XL GROWING PARAMETERS FOR VARIOUS MUSHROOM SPECIES

XII. CULTIVATION PROBLEMS AND THEIR SOLUTIONS: A TROUBLESHOOTING GUIDE

XIII. THE CONTAMINANTS OF MUSHROOM CULTURE: IDENTIFICATION AND CONTROL

XIV. THE PESTS OF MUSHROOM CULTURE

XV. MUSHROOM GENETICS

APPENDICES

GLOSSARY

BIBLIOGRAPHY

INDEX

PHOTOGRAPHY AND ILLUSTRATION CREDITS

ACKNOWLEDGEMENTS

OCR
Sterile Technique and Agar Culture/27
cultures from wild specimens may fruit very poorly in an artificial environment. Just as with wild
plants, strains of wild mushrooms must be selectively developed.
Of the many newly created strains intrinsic to multispore germination, some may be only capable of vegetative growth. Such mycelia can assimilate nutrients but can not form a mushroom fruitbody (the product of generative growth). A network of cells coming from a single spore is called a
monokaryon. As a rule, monokaryons are not capable of producing fertile spore-bearing mushrooms. When two compatible monokaryons encounter one another and mate, cytoplasmic and ge-

netic material is exchanged. The resultant mycelium is a dikaryon that can produce fertile offspring in the form of mushrooms. Branching or networking between different dikaryotic strains is
known as anastomosis. This process of recombination can occur at any stage of the cultivation
process: on agar; on grain; or on bulk substrates. The crossing of different mushroom strains is analogous to the creation of hybrids in horticulture.
Another method for starting cultures is the creation of single spore isolates and is accomplished
by diluting spores in a volume of sterile water. This spore solution is further diluted into larger volumes of sterile water which is in turn used to inoculate media dishes. In this way, cultivators can ob-

Figure 28 Four strains of Psilocybe cubensis mycelium: (clockwise, upper right) Matias
Romero; Misantla; Amazonian; and Palenque.

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