The mushroom cultivator. A practical guide to growing mushrooms at home

Paul Stamets. The mushroom cultivator. A practical guide to growing mushrooms at home. - Agarikon press, 1983

Содержание

FOREWORD by Dr. Andrew Weil

PREFACE

I. INTRODUCTION TO MUSHROOM CULTURE

II. STERILE TECHNIQUE AND AGAR CULTURE

III. GRAIN CULTURE

IV. THE MUSHROOM GROWING ROOM

V. COMPOST PREPARATION

VI. NON-COMPOSTED SUBSTRATES

VII. SPAWNING AND SPAWN RUNNING IN BULK SUBSTRATES

VIII. THE CASING LAYER

IX. STRATEGIES FOR MUSHROOM FORMATION (PINHEAD INITIATION)

X. ENVIRONMENTAL FACTORS: SUSTAINING THE MUSHROOM CROP

XL GROWING PARAMETERS FOR VARIOUS MUSHROOM SPECIES

XII. CULTIVATION PROBLEMS AND THEIR SOLUTIONS: A TROUBLESHOOTING GUIDE

XIII. THE CONTAMINANTS OF MUSHROOM CULTURE: IDENTIFICATION AND CONTROL

XIV. THE PESTS OF MUSHROOM CULTURE

XV. MUSHROOM GENETICS

APPENDICES

GLOSSARY

BIBLIOGRAPHY

INDEX

PHOTOGRAPHY AND ILLUSTRATION CREDITS

ACKNOWLEDGEMENTS

OCR
20/The Mushroom Cultivator
range. One needs to be concerned with exact pH levels only if spores fail to germinate or if mycelial
growth is unusually slow.

What follows are several formulas for the preparation of nutritionally balanced enriched agar

media, any one of which is highly suited for the growth of Agaricus, Pleurotus, Len linus,
Stropharia, Lepista, Flammulina, Volvariella, Panaeolus and Psilocybe mycelia. Of these the
authors have two preferences: PDY (Potatoe Dextrose Yeast) and MPG (Malt Peptone Grain) agar
media. The addition of ground rye grain or grain extract to whatever media is chosen clearly promotes the growth of strandy mycelium, the kind that is generally preferred for its fast growth.
Choose one formula, mix the ingredients in dry form, place into a flask and add water until one
liter of medium is made.

PDY (Potato Dextrose Yeast) Agar
the filtered, extracted broth from boiling
300 grams of sliced potatoes in liter of
water for 1 hour
1

10 grams dextrose sugar
2 grams yeast (optional)
20 grams agar

MPG (Malt Peptone Grain) Agar
20 grams tan malt
5 grams ground rye grain
5 grams peptone or neopeptone
2 grams yeast (optional)
20 grams agar

MEA (Malt Extract Agar)
20 grams tan malt
2 grams yeast
20 grams agar

(Avoid dark brewer s malts which have
become carmellized. The malt that
should be used is a light tan brewer's
malt which is powdery, not sticky in
form)

For controlling bacteria, 0.10 grams of 60-80% pure gentamycin sulfate can be added to each
liter of media prior to sterilization. (See Resources in Appendix.)

Water quality—its pH and mineral content—varies from region to region. If living in an area of
questionable water purity, the use of distilled water is advisable. For all practical purposes, however,
tap water can be used without harm to the mushroom mycelium. A time may come when balancing

pH is important—especially at spore germination or in the culture of exotic species. The pH of
media can be altered by adding a drop at a time of 1 molar concentration of hydrochloric acid
(HCL) or sodium hydroxide (NaOH). The medium is thoroughly mixed and then measured using a
pH meter or pH papers. (One molar HCL has a pH of 0; one molar NaOH has a pH of 1 2; and
distilled water has a pH of 7).
After thoroughly mixing these ingredients, sterilize the medium in a pressure cooker for 30 minutes at 1 5 psi. (Pressure cookers are a safe and effective means of sterilizing media provided they

are operated according to the manufacturer's instructions). A small mouthed vessel is recommended for holding the agar media. If not using a flask specifically manufactured for pouring media,
any narrow necked glass bottle will suffice. Be sure to plug its opening with cotton and cover with

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