The mushroom cultivator. A practical guide to growing mushrooms at home

Paul Stamets. The mushroom cultivator. A practical guide to growing mushrooms at home. - Agarikon press, 1983

Содержание

FOREWORD by Dr. Andrew Weil

PREFACE

I. INTRODUCTION TO MUSHROOM CULTURE

II. STERILE TECHNIQUE AND AGAR CULTURE

III. GRAIN CULTURE

IV. THE MUSHROOM GROWING ROOM

V. COMPOST PREPARATION

VI. NON-COMPOSTED SUBSTRATES

VII. SPAWNING AND SPAWN RUNNING IN BULK SUBSTRATES

VIII. THE CASING LAYER

IX. STRATEGIES FOR MUSHROOM FORMATION (PINHEAD INITIATION)

X. ENVIRONMENTAL FACTORS: SUSTAINING THE MUSHROOM CROP

XL GROWING PARAMETERS FOR VARIOUS MUSHROOM SPECIES

XII. CULTIVATION PROBLEMS AND THEIR SOLUTIONS: A TROUBLESHOOTING GUIDE

XIII. THE CONTAMINANTS OF MUSHROOM CULTURE: IDENTIFICATION AND CONTROL

XIV. THE PESTS OF MUSHROOM CULTURE

XV. MUSHROOM GENETICS

APPENDICES

GLOSSARY

BIBLIOGRAPHY

INDEX

PHOTOGRAPHY AND ILLUSTRATION CREDITS

ACKNOWLEDGEMENTS

OCR
Sterile Technique and Agar Culture/19

PREPARATION OF AGAR MEDIA
Once the sterile laboratory is completed, the next step is the preparation of nutrified agar media. Derived from seaweed, agar is a solidifying agent similar to but more effective than gelatin.
There are many recipes for producing enriched agar media suitable for mushroom culture. The
standard formulas have been Potatoe Dextrose Agar (PDA) and Malt Extract Agar (MEA) to which
yeast is often added as a nutritional supplement. Many of the mycological journals list agar media
containing peptone or neopeptone, two easily accessed sources of protein for mushroom mycehum. Another type of agar media that the authors recommend is a broth made from boiling wheat
or rye kernels which is then supplemented with malt sugar.
If a high rate of contamination from bacteria is experienced, the addition of antibiotics to the
culture media will prevent their growth. Most antibiotics, like streptomycin, are not autoclavable and
must be added to the agar media after sterilization while it is still molten. One antibiotic, gentamycin
sulfate, survives autoclaving and is effective against a broad range of bacteria. Antibiotics should be
used sparingly and only as a temporary control until the sources of bacteria can be eliminated. The
mycehia of some mushroom species are adversely affected by antibiotics.
Dozens of enriched agar media have been used successfully in the cultivation of fungi and
every cultivator develops distinct preferences based on experience. Regardless of the type of agar
medium employed, a major consideration is its pH, a logarithmic scale denoting the level of acidity
or alkalinity in a range from 0 (highly acidic) to 1 4 (highly basic) with 7 being neutral. Species of
Psilocybe thrive in media balanced between 60-7.0 whereas Agaricus brunnescens and allies
grow better in near neutral media. Most mycelia are fairly tolerant and grow well in the 5.5-7.5 pH

Figure 21
Standard
glove box.

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