Growing gourmet and medical mushrooms

Paul Stamets. Growing gourmet and medical mushrooms. - Ten Speed Press, 2000


1. Mushrooms, Civilization and History

2. The Role of Mushrooms in Nature

3.Selecting a Candidate for Cultivation

4. Natural Culture: Creating Mycological Landscapes

5. The Stametsian Model: Permaculture with a Mycological Twist

6. Materials fo rFormulating a Fruiting Substrate

7. Biological Efficiency: An Expression of Yield

8. Home-made vs. Commercial Spawn

9. The Mushroom Life Cycle

10. The Six Vectors of Contamination

11. Mind and Methods for Mushroom Culture

12. Culturing Mushroom Mycelium on Agar Media

13. The Stock Culture Library: A Genetic Bank of Mushroom Strains

14. Evaluating a Mushroom Strain

15. Generating Grain Spawn

16. Creating Sawdust Spawn

17. Growing Gourmet Mushrooms on Enriched Sawdust

18. Cultivating Gourmet Mushrooms on Agricultural Waste Products

19. Cropping Containers

20. Casing: A Topsoil Promoting Mushroom Formation

21. Growth Parameters for Gourmet and Medicinal Mushroom Species

Spawn Run: Colonizing the Substrate

Primordia Formation: The Initiation Strategy

Fruitbody (Mushroom) Development

The Gilled Mushrooms

The Polypore Mushrooms of the Genera Ganoderma, Grifola and Polyporus

The Lion’s Mane of the Genus Hericium

The Wood Ears of the Genus Auricularia

The Morels: Land-Fish Mushrooms of the Genus Morchella

The Morel Life Cycle

22. Maximizing the Substrate’s Potential through Species Sequencing

23. Harvesting, Storing, and Packaging the Crop for Market

24. Mushroom Recipes: Enjoying the Fruits of Your Labors

25. Cultivation problems & Their Solutions: A Troubleshoting guide


I. Description of Environment for a Mushroom Farm

II. Designing and Building A Spawn Laboratory

III. The Growing Room: An Environment for Mushroom Formation & Development

IV. Resource Directory

V. Analyses of Basic Materials Used in Substrate Preparation

VI. Data Conversion Tables





exponential expansion of mycelial mass. The
simplest method for most cultivators to follow
is similar to the classic spawn expansion schedule employed by most laboratories. The first
stage is to grow the mycelium on nutrified agar
media in petri dishes. The next is transferring
pure cultures onto sterilized grain, typically in
jars (quart, half gallons, gallons).At75° F. (24°
C.), two to three weeks pass before colonization is complete. Each of these "Grain
Masters" can inoculate 10 gallon jars each con-

Figure 331. Brown spores, although released from
the underside, tend to collect on the upper cap surfaces of G. lucidum.

taining 1000-1200 grams of sterilized rye
grain. Once grown out, each gallon jar of
spawn can readily inoculate 10 5 lb. bags of

hardwood sawdust (wet weight, 65-70% moisture). The resulting sawdust spawn is the last
step before inoculating a substrate capable of
supporting fruitbodies.
are: to grow fruitbodies on sawdust/chips;
At this juncture, the cultivator
fruit Ganoderma lucidum on vertically
to grow fruitbodies on buried logs; to inoculate stumps; or to
under development by the
arranged columns of heat treated sawdust. (This last method is currently
agar-to-grain-to-sawdustauthor.) These four strategies all follow the same expansion schedule
to the production block.

Uninhibited by gentamycin
Suggested Agar Culture Media: MEA. OMYA, PDYA and/or DFA.
sulfate (1/15th gram/liter.)

cereal grains. Fruitbodies do not form
fans of growth climb the inside surfaces
on most grains except milo (a type of sorghum), whereupon
used directly after colonization,
of the spawn containers and fruit within. If mature grain spawn is not
shaking. In this case, a
over-incubation results, making it difficult to disperse the grain kernels upon
apart the spawn within
transfer tool such as a sterilized spoon, knife, or similar tool is needed to

1st Generation Spawn Media: Rye grain, wheat grain, other

the jar.

that described in The
I prefer the liquid inoculation method of generating spawn similar to

mycelium of this species readily
Mushroom Cultivator by Stamets & Chilton (1983). Further, the
of Ganoderrna lucidum
adapts to submerged fermentation. Submerged fermentation (liquid culture)
mycelium is considered "traditional" in China.
be expanded (3 cups
2nd & 3rd Generation Spawn Media: Each unit of primary spawn can
moist sawdust. Once inoculated,
grain in 1/2 gallon jar) into 10 (5-20) units of 5 lbs. sterilized,
days. The 10 sawdust spawn
and incubated at 75 ° F. (24° C.) colonization is complete
in turn, are colonized in a
blocks can be expanded into 100-200 3-5 lbs. sawdust/chip bags, which
similar period of time.

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