Growing gourmet and medical mushrooms

Paul Stamets. Growing gourmet and medical mushrooms. - Ten Speed Press, 2000


1. Mushrooms, Civilization and History

2. The Role of Mushrooms in Nature

3.Selecting a Candidate for Cultivation

4. Natural Culture: Creating Mycological Landscapes

5. The Stametsian Model: Permaculture with a Mycological Twist

6. Materials fo rFormulating a Fruiting Substrate

7. Biological Efficiency: An Expression of Yield

8. Home-made vs. Commercial Spawn

9. The Mushroom Life Cycle

10. The Six Vectors of Contamination

11. Mind and Methods for Mushroom Culture

12. Culturing Mushroom Mycelium on Agar Media

13. The Stock Culture Library: A Genetic Bank of Mushroom Strains

14. Evaluating a Mushroom Strain

15. Generating Grain Spawn

16. Creating Sawdust Spawn

17. Growing Gourmet Mushrooms on Enriched Sawdust

18. Cultivating Gourmet Mushrooms on Agricultural Waste Products

19. Cropping Containers

20. Casing: A Topsoil Promoting Mushroom Formation

21. Growth Parameters for Gourmet and Medicinal Mushroom Species

Spawn Run: Colonizing the Substrate

Primordia Formation: The Initiation Strategy

Fruitbody (Mushroom) Development

The Gilled Mushrooms

The Polypore Mushrooms of the Genera Ganoderma, Grifola and Polyporus

The Lion’s Mane of the Genus Hericium

The Wood Ears of the Genus Auricularia

The Morels: Land-Fish Mushrooms of the Genus Morchella

The Morel Life Cycle

22. Maximizing the Substrate’s Potential through Species Sequencing

23. Harvesting, Storing, and Packaging the Crop for Market

24. Mushroom Recipes: Enjoying the Fruits of Your Labors

25. Cultivation problems & Their Solutions: A Troubleshoting guide


I. Description of Environment for a Mushroom Farm

II. Designing and Building A Spawn Laboratory

III. The Growing Room: An Environment for Mushroom Formation & Development

IV. Resource Directory

V. Analyses of Basic Materials Used in Substrate Preparation

VI. Data Conversion Tables






Growth Parameters
Spawn Run:
Incubation Temperature: 70-80° F. (2 1-27° C.)
Relative Humidity: 95-100%
Duration: 25-45 days
C02: > 20,000 ppm
Fresh Air Exchanges: 0-1 per hour
Light Requirements: n/a

Primordia Formation:
InitiationTemperature: 50-60° F. (10-16° C.)
Relative Humidity: 95-98%
Duration: 14-2 1 days
C02: <1500 ppm
Fresh Air Exchanges: 4-8 per hour or as needed to affect CO2
Light Requirements: 100-500 lux.

Fruitbody Development:
IncubationTemperature: 60-70° F. (16-21° C.)
Relative Humidity: (85) 90-95%
Duration: 7-14 days
C02: < 1500 ppm
FreshAir Exchanges: 4-8 per hour
Light Requirements: 100-500 lux.

Cropping Cycle:
2 crops, 3-4 weeks apart.

SuggestedAgar Media: MYA, PDYA, OMYA or DFA.

1st Generation Spawn Media: Grain (rye, wheat, milo, sorghum, or corn) should be inoculated
from 3-4 day fermented mycelium. (A high inoculation rate is required if not using liquid inoculation
techniques.) Spawn jars should be shaken every 3-4 days after inoculation to ensure full colonization.
On sterilized grain, after being disturbed, the mycelium of this species revitalizes, bursting into new

2nd & 3rd Generation Spawn Media: A second generation of grain spawn can be inoculated from
the 1St generation and then the grain spawn can be mixed into a moistened 50:5 0 sawdust/chips blend.
A minimum of a 10% inoculation rate should be used, or preferably a rate of 15-20%.

Fruiting Substrates: For indoor cultivation, pasteurized straw or sterilized sawdust is inoculated
with grain spawn and incubated under high carbon dioxide conditions. Once colonized, a microbially
rich soil (a "casing layer") is placed upon it to promote fruiting. This soil can be heat treated to kill
insects but should not be exposed to temperatures higher than 140° F. (60° C.) for more than one hour.

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