Growing gourmet and medical mushrooms

Paul Stamets. Growing gourmet and medical mushrooms. - Ten Speed Press, 2000


1. Mushrooms, Civilization and History

2. The Role of Mushrooms in Nature

3.Selecting a Candidate for Cultivation

4. Natural Culture: Creating Mycological Landscapes

5. The Stametsian Model: Permaculture with a Mycological Twist

6. Materials fo rFormulating a Fruiting Substrate

7. Biological Efficiency: An Expression of Yield

8. Home-made vs. Commercial Spawn

9. The Mushroom Life Cycle

10. The Six Vectors of Contamination

11. Mind and Methods for Mushroom Culture

12. Culturing Mushroom Mycelium on Agar Media

13. The Stock Culture Library: A Genetic Bank of Mushroom Strains

14. Evaluating a Mushroom Strain

15. Generating Grain Spawn

16. Creating Sawdust Spawn

17. Growing Gourmet Mushrooms on Enriched Sawdust

18. Cultivating Gourmet Mushrooms on Agricultural Waste Products

19. Cropping Containers

20. Casing: A Topsoil Promoting Mushroom Formation

21. Growth Parameters for Gourmet and Medicinal Mushroom Species

Spawn Run: Colonizing the Substrate

Primordia Formation: The Initiation Strategy

Fruitbody (Mushroom) Development

The Gilled Mushrooms

The Polypore Mushrooms of the Genera Ganoderma, Grifola and Polyporus

The Lion’s Mane of the Genus Hericium

The Wood Ears of the Genus Auricularia

The Morels: Land-Fish Mushrooms of the Genus Morchella

The Morel Life Cycle

22. Maximizing the Substrate’s Potential through Species Sequencing

23. Harvesting, Storing, and Packaging the Crop for Market

24. Mushroom Recipes: Enjoying the Fruits of Your Labors

25. Cultivation problems & Their Solutions: A Troubleshoting guide


I. Description of Environment for a Mushroom Farm

II. Designing and Building A Spawn Laboratory

III. The Growing Room: An Environment for Mushroom Formation & Development

IV. Resource Directory

V. Analyses of Basic Materials Used in Substrate Preparation

VI. Data Conversion Tables




An alternative method for generating spawn
is via Liquid Culture. This method saves time,
money, and is less susceptible to contamination.
These techniques are described further on.
The next step is for each of theseThird Generation spawn units to inoculate ten to twenty
its mass in sawdust or straw. See Chapters 16
and 17.

Autoclavable Spawn Bags
Autoclavable bags have been used by the
mushroom industry for nearly 40 years. Primary uses for autoclavable bags are for the
incubation of grain and sawdust. Preferences
vary widely between cultivators. Flat, nongussetted bags are popular for incubating grain

spawn. The more grain filled into a bag, the
greater the danger of poor gas exchange, a mafactor leading to contamination.
Three-dimensional gusseted bags are used pri-

Figure 113. Gallon jars of 3rd generation grain
spawn incubating.

less contaminated, these terminal spawnjars usually are of sufficient quality for inoculating bulk

substrates. Of course, the spawn manager can
always exercise the option of using First, Second

or Third Generation grain spawn for inoculating sawdust or straw.
In effect, the spawn maker has taken a single
petri dish and in three generations of transfers
created 250 gallons of spawn .Therefore, a stack
of twenty petri dishes can give rise to 5000 gal-

lons of spawn! This places a whole new
perspective on the sheer biological power inher-

ent within a single test tube slant, which can
easily inoculate a sleeve of 20 petri dishes. Most
laboratories do not fully realize the potential of
every culture. In many cases, spawn expansion

is terminated at G2. Many spawn managers
choose not to "chase" the optimum. Few lab oratories are large enough to accommodate the
end result of the methods described here.

Figure 114. Grain spawn ready for use.

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