Growing gourmet and medical mushrooms

Paul Stamets. Growing gourmet and medical mushrooms. - Ten Speed Press, 2000


1. Mushrooms, Civilization and History

2. The Role of Mushrooms in Nature

3.Selecting a Candidate for Cultivation

4. Natural Culture: Creating Mycological Landscapes

5. The Stametsian Model: Permaculture with a Mycological Twist

6. Materials fo rFormulating a Fruiting Substrate

7. Biological Efficiency: An Expression of Yield

8. Home-made vs. Commercial Spawn

9. The Mushroom Life Cycle

10. The Six Vectors of Contamination

11. Mind and Methods for Mushroom Culture

12. Culturing Mushroom Mycelium on Agar Media

13. The Stock Culture Library: A Genetic Bank of Mushroom Strains

14. Evaluating a Mushroom Strain

15. Generating Grain Spawn

16. Creating Sawdust Spawn

17. Growing Gourmet Mushrooms on Enriched Sawdust

18. Cultivating Gourmet Mushrooms on Agricultural Waste Products

19. Cropping Containers

20. Casing: A Topsoil Promoting Mushroom Formation

21. Growth Parameters for Gourmet and Medicinal Mushroom Species

Spawn Run: Colonizing the Substrate

Primordia Formation: The Initiation Strategy

Fruitbody (Mushroom) Development

The Gilled Mushrooms

The Polypore Mushrooms of the Genera Ganoderma, Grifola and Polyporus

The Lion’s Mane of the Genus Hericium

The Wood Ears of the Genus Auricularia

The Morels: Land-Fish Mushrooms of the Genus Morchella

The Morel Life Cycle

22. Maximizing the Substrate’s Potential through Species Sequencing

23. Harvesting, Storing, and Packaging the Crop for Market

24. Mushroom Recipes: Enjoying the Fruits of Your Labors

25. Cultivation problems & Their Solutions: A Troubleshoting guide


I. Description of Environment for a Mushroom Farm

II. Designing and Building A Spawn Laboratory

III. The Growing Room: An Environment for Mushroom Formation & Development

IV. Resource Directory

V. Analyses of Basic Materials Used in Substrate Preparation

VI. Data Conversion Tables






Figure 62. Diagrammatic representation of the effectiveness of filtration media. Dirty air is first filtered
through a coarse prefilter (30% @ 10 j.t), then an electrostatic filters (99% @ 1 g), and finally through a High
Efficiency Particulate Air (HEPA) filter (99.99% @ .3 it). Only some free-flying endospores of bacteria and
viruses pass through.

The monumental task of creating a sterile environment has been difficult, until recently. The
invention of high efficiency particulate air filters (called HEPA filters) has made sterile tissue

culture achieveable to all. In vitro ("within
glass") propagation of plants, animals, fungi,

bacteria, protozoa became commercially pos-

sible. Today, the HEPA (High Efficiency
Particulate Air) filter is by far the best filtration
system in use, advancing the field of tissue culture more-so than any other invention. ** When
air is forcibly pressed through these filters, all
contaminants down to .3 microns (p) are elimi-

nated with a 99.99% efficiency. This means

Scientists have recently discovered a group of heat resistant

bacteria thriving in the fumaroles of submerged, active volcanoes.These bacteria thrive where no other life forms live.

Many types of filtration systems are available. Ionizers, for
our purposes, are insufficient in their aircleaning capacity. For
a comparison of filtration systems, which rates HEPA filtration as the best, please refer to Consumer Reports, Oct.1992,
pg. 657.A new generation of micron filters, the ULPA filters
screen out particulates down to .1 microns with a 99.9999%
efficiency. This means only I particle measuring .1 microns in
diameter of every 1,000,000 flows through the filtration medium.

only 1 of every 10,000 particulates exceeding.
3 p pass through. For all practical purposes, a
sterile wind is generated downstream from the

filter. The cultivator works within this airstream. This unique environment calls for
unique techniques .A different set of rules now
presides, the violation of which invites disaster.

Sterile tissue culture technique fails if it
solely relies on mechanical means. Sterile tis-

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